qPCR and gene expression
Primer pairs, hydrolysis probes, reference gene assays.
- Primer pair + probe set
- Dual-labeled probe
- Double-quenched probe
- Reference gene assay
Cloning and sequencing
Cloning primers, seamless assembly overhangs, universal vector primers.
- 2 OD desalted primer
- 5 OD, for a primer you will use all year
- Assembly primer over 60 nt, quoted individually
Gene silencing
siRNA duplexes, miRNA mimics and inhibitors, matched controls.
- siRNA duplex
- miRNA mimic
- Scrambled control
Genome editing
crRNA with tracrRNA, ssODN donors, validation primers.
- crRNA
- tracrRNA Quoted
- Non-targeting control
Genotyping and SNP
Allele-specific primers, genotyping probe pairs, blockers.
- Two probes, two dyes
- Allele-specific primers
Imaging and hybridisation
FISH probes, molecular beacons, labelled oligos.
- Molecular beacon
- Single-dye labelled oligo
Conjugation and biosensing
Thiol, amino, biotin and click chemistry oligos.
- 5′ biotin, HPLC
- 5′ amino C6, HPLC
- 5′ thiol C6, HPLC
Plates and large scale
96 and 384-well plates, normalised delivery, bulk synthesis.
- 10 OD delivered
- 96-well plate, normalised, quoted individually
Not sure which is right? Describe the experiment and we will propose a design with the
reasoning, free of charge. Start from a gene.